Chapter 7 Methods in Avian Embryology Experimental and Molecular Manipulation of the Embryonic Chick Limb
Publisher SummaryThis chapter presents methods in avian embryology experimental and molecular manipulation of the embryonic chick limb. The developing limb appendage, from a vertebrate or an invertebrate animal, studies many questions in developmental biology such as pattern formation, skeletal development, cell fate, morphogenesis, signaling, gene regulation, and cell and molecular interactions. Limb development can be perturbed without affecting the development of other tissues or the animal itself and hence, provides a simple system to test the function of genes that may be required more generally during embryonic development. Focusing on the developing chick limb, classical experimental embryology studies discuss the critical organizing centers that govern the three axes of the limb—namely, proximal–distal (Pr–D; shoulder to tips of digits), anterior–posterior (A–P; thumb to little finger), and dorsal– ventral (D–V; knuckle to palm). The experiments that lead to these Read more...
Publication date: 2008/01/01