Biography:

In the past T.B. Tennikova has collaborated on articles with E.G. Vlakh and A.Yu. Menshikova. One of their most recent publications is High-performance membrane chromatography of proteins, a novel method of protein separation. Which was published in journal Journal of Chromatography A.

More information about T.B. Tennikova research including statistics on their citations can be found on their Copernicus Academic profile page.

T.B. Tennikova's Articles: (7)

High-performance membrane chromatography of proteins, a novel method of protein separation

AbstractMacroporous polymeric membranes, approximately 1 mm thick, were synthesized from poly(glycidyl methacrylate-co-ethylene dimethacrylate). The epoxide groups were further derivatized to add different functional groups which are commonly used as stationary phases in comumn chromatography. Separations of model mixutres of proteins were carried out on membranes modified with sulpho, C4 or C8 groups; the results were similar to those obtained using a column separation in the ion-exchange mode and in hydrophobic interaction chromatography. The advantage of high-performance membrane chromatography (HPMC) is that the pressure used is lower by as much as two orders of magnitude than that required to reach the same flow-rate in a packed column, although a high loading capacity can still be achieved. This makes HPMC suitable for both analytical and preparative separations.

Phase transition chromatography of polyesters on macroporous glycidyl methacrylate—ethylene dimethacrylate copolymers

AbstractThe chromatographic behaviour of oligoesters of phenolphthalein and terephthalic acid in columns packed with a polymeric sorbent based on the macroporous strongly cross-linked copolymer glycidyl methacrylate—ethylene dimethacrylate was studied. The possibility of the effective separation of oligomers according to the type of terminal groups was demonstrated. For comparison the sorbent Silasorb 600 was used.

ReviewApplications of polymethacrylate-based monoliths in high-performance liquid chromatography

AbstractMonolithic columns were introduced in the early 1990s and have become increasingly popular as efficient stationary phases for most of the important chromatographic separation modes. Monoliths are functionally distinct from porous particle-based media in their reliance on convective mass transport. This makes resolution and capacity independent of flow rate. Monoliths also lack a void volume. This eliminates eddy dispersion and permits high-resolution separations with extremely short flow paths. The analytical value of these features is the subject of recent reviews. Nowadays, among other types of rigid macroporous monoliths, the polymethacrylate-based materials are the largest and most examined class of these sorbents. In this review, the applications of polymethacrylate-based monolithic columns are summarized for the separation, purification and analysis of low and high molecular mass compounds in the different HPLC formats, including micro- and large-scale HPLC modes.

Monodisperse carboxylated polystyrene particles: synthesis, electrokinetic and adsorptive properties

AbstractEffect of conditions of styrene dispersion polymerization initiated by 4,4′-azo-bis-(4-cyanopentanoic acid) in ethanol solutions of polyvinylpyrrolidone was investigated. Suitable methods ensuring the control of final particle size, surface structure, and surface concentration of carboxylic groups in the polymerization process are discussed. Particle ability to interact with protein was also studied. Monodisperse particles of diameters up to 4 μm had a complex surface layer containing polyvinylpyrrolidone-graft-polystyrene copolymers as well as carboxylic groups of the initiator. The effect of this surface structure on the isotherms of adsorption and chemisorption of bovine serum albumin was revealed. Electrophoretic mobility of the particles and their isoelectric point values before and after protein binding depending on pH and ionic strength were determined. These data depend on conditions of particle preparation as well as on protein coating values.

Hydrophilic methacrylate monoliths as platforms for protein microarray

AbstractHydrophilic macroporous monolithic materials based on a copolymer of 2-hydroxyethyl methacrylate with glycerol dimethacrylate was synthesized by photo-initiated free-radical polymerization of monomers in a presence of the low molecular mass porogens, such as cyclohexanol, dodecanol, toluene and heptane, as well as the solutions of hydrophobic polymers, namely, polystyrene of different molecular weights and concentrations in toluene, and poly(dimethyl siloxane) in heptane. The Hildebrand solubility parameters were used to predict the diluent-polymer compatibility. Pore size distribution and surface area characterization have been assessed by mercury intrusion porosimetry; scanning electron microscopy was used to evaluate the differences in macroporous morphology obtained with different porogenic agents. The monolithic materials were covalently attached to a glass surface directly at polymerization step. Monolithic layers were applied as platforms for microarrays to accomplish highly sensitive solid-phase protein analysis. The efficiency of developed microarrays was demonstrated using mouse IgG and goat anti-mouse IgG as a model affinity pair.

New platforms for 3-D microarrays: Synthesis of hydrophilic polymethacrylate monoliths using macromolecular porogens

AbstractHydrophilic macroporous monolithic material based on poly(glycidyl methacrylate-co-glycerol dimethacrylate) was synthesized by photo-initiated free-radical polymerization. Different pore-forming agents, including low molecular mass cyclohexanol and dodecanol, as well as the solutions of hydrophobic polymers, namely, polystyrene in toluene and poly(dimethyl siloxane) in hexane, were used to obtain the macroporous polymer platforms intended for new type of 3-D microarrays (biochips). The porous characteristics of functional copolymers obtained were investigated by mercury intrusion porosimetry and scanning electron microscopy. Some of developed materials were tested for protein microarray construction. Demonstration of potential of suggested materials, as well as optimization of protein covalent immobilization conditions, were realized using model mouse IgG – goat anti-mouse IgG affinity pair.

Self-assembled spin-labeled nanoparticles based on poly(amino acids)

AbstractThe development of detectable nanoparticles for controlled drug delivery systems has tremendous practical importance regarding the monitoring of drug pathway in organism. Self-assembly amphiphilic block-copolymer poly(l-glutamic acid)-b-poly(l-phenylalanine) (pGlu-b-pPhe) was chosen for the preparation of discussed nanoparticles. The synthesis of blocks was carried out using ring-opening polymerization (ROP) of N-carboxyanhydrides of mentioned amino acids. To introduce the spin label at C-terminal position of hydrophilic block, (4-amino-2,2,6,6-tetramethylpiperidin-1-yl)oxyl (4-amino-TEMPO) was applied as ROP initiator and the polymerization of hydrophobic block was carried out with previously synthesized macroinitiator. The results obtained by transmission electron microscopy clearly showed that TEMPO-pGlu-b-pPhe polymer was really capable to self-assembling in aqueous solutions followed by polymersome formation. The mean size of nanoparticles was increased in a range of TEMPO-pGlu43-b-pPhe12 < TEMPO-pGlu43-b-pPhe29 < TEMPO-pGlu43-b-pPhe49 as 60 < 200 < 280 nm, respectively. EPR spectroscopy of the solutions of spin-labeled homopolymer TEMPO-p-γ-Glu(Bzl), block copolymers TEMPO-p-γ-Glu(Bzl)-b-pPhe and suspension of polymersomes formed from TEMPO-p-Glu-b-pPhe was performed and the results were compared. It was proved that in the case of nanoparticles EPR detectable spin labels are located on polymersome surface. The experiments in cell culture demonstrated the absence of cytotoxicity of labeled nanoparticles. Additionally, it was shown that TEMPO-label can be detected inside the cell by EPR method.

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