Biography:

One of their most recent publications is Studies on the mechanism of cortisol inhibition of human natural killer cell activity: Effects of calcium entry blockers and calmodulin antagonists. Which was published in journal Steroids.

More information about Giovanni Gatti research including statistics on their citations can be found on their Copernicus Academic profile page.

Giovanni Gatti's Articles: (1)

Studies on the mechanism of cortisol inhibition of human natural killer cell activity: Effects of calcium entry blockers and calmodulin antagonists

AbstractThe role of Ca2+ in mediating the inhibition by glucocorticoids of human natural killer (NK) activity was investigated using Ca2+ entry blockers (verapamil and its desmethoxy-derivatives LU46973 and LU47093) and calmodulin antagonists (pimozide and two naphthalenesulfonamide derivatives, W-7 and W-13). Peripheral blood mononuclear (PBM) cell preparations were incubated for 20 h with 1×10−6 M Cortisol and these agents in various combinations (concentration range: 1×10−7 − 1×10−5 M) and then assayed in a direct 4-h cytolytic assay using 51Cr-labeled K 562 target cells. Exposure to cortisol led to a significant reduction of NK cell activity (about 50% with respect to the spontaneous activity). Ca2+ entry blockers displayed per se a dose-dependent depressive effect on cytotoxicity and gave significant enhancement of cortisol-dependent inhibition. Calmodulin antagonists were per se minimally effective but clearly amplified the cortisol-mediated inhibition. Raising extracellular Ca2+ by CaCl2 or intracellular Ca2+ by the ionophore A23187 yelded an appreciable reduction of these effects. Our data are compatible with the view that extracellular and intracellular Ca2+ play a role in the control of human NK cell activity. Moreover, it is conceivable that the mechanisms involved in glucocorticoid inhibition of NK cell activity involve Ca2+-dependent pathways.

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