Highlights•R-IM exerted a stronger inhibitory effect on wheat growth than S-IM.•R-IM induced more oxidative stress in wheat than S-IM.•R-IM inhibited antioxidant enzyme gene expression more strongly than S-IM.•From the metabolic level, R-IM disturbed more metabolic pathways than S-IM.
Highlights•The crystal lattice, band gap and band edges of layered MoO3 are reproduced.•The electronic structures of MoO3 are predominated by the in-plane atomic orbitals.•The anisotropic optical adsorption sources from state-resolved electrons near VBM.
AbstractIn this work, a kind of preparation method of zwitterionic ion chromatography (ZIC) stationary phase modified with phosphorylcholine (PC) was obtained by hydrolyzing after bonding phosphorylcholine dichloride to diol-silica to better explore the characteristics of the PC groups as ZIC stationary phase ligand in simultaneous separation of acidic proteins and basic proteins. The results showed that two kinds of acidic proteins and three kinds of basic proteins can be separated completely, meanwhile, hen egg white was separated and purified and three kinds of egg white components ovalbumin, G2 ovoglobulin and ovotransfemin proteins were separated completely by one single step on PC-ZIC column, the purity of all proteins reached above 95%. PC-ZIC stationary phase was successfully improved with better separation capacity and selectivity than previously reported in this paper.