Biography:

In the past Ciro Esposito has collaborated on articles with Giulio Tarro and Carla Esposito. One of their most recent publications is Further studies on an immunoenzymatic assay for herpes simplex virus tumor-associated antigen. Which was published in journal Clinical Immunology and Immunopathology.

More information about Ciro Esposito research including statistics on their citations can be found on their Copernicus Academic profile page.

Ciro Esposito's Articles: (12)

Further studies on an immunoenzymatic assay for herpes simplex virus tumor-associated antigen

AbstractAn immunoenzymatic assay (ELISA) has been developed to detect specific antibodies for herpes simplex virus (HSV) type 1 and 2 tumor-associated antigen (TAA) in sera of patients with head, neck, and urogenital tract carcinomas. Further tests with coded sera have now shown that 2185 controls (24.70%), i.e., normal people matched by age or affected with benign conditions, are positive against 4571 patients (with HSV-associated tumors (63.38%). Seven of twenty people with rectum-colon cancers are positive (35.00%). Immunodepression and chemo- and radiotherapy play an inhibitory role on the positivity of ELISA for HSV-TAA. Radical surgery of the positive cancers results in loss of specific antibody whereas relapses or metastasis of the tumor yields positive results. The reproducibility of the test is good and the standard error is low. The use of this ELISA for detection of HSV-TAA antibodies in certain human carcinomas is discussed.

NoteSerological and virological investigations of young children with acute respiratory syndrome associated with respiratory syncytial virus

AbstractFrom January 1979 to March 1979, 341 young children from the metropolitan area of Naples, Italy, were hospitalized with respiratory virus disease. Diagnosis of patients made from virus isolation and seroconversion indicate that the respiratory syncytial virus was a primary cause of this acute respiratory syndrome.

Regular ArticleOverlapping between Fluorescence Modifications and Activation of Prostate Transglutaminase Induced by Sodium Dodecyl Sulfate☆☆☆

AbstractThe transglutaminase from rat coagulating gland secretion has been proposed as a new member of the transglutaminase family. Its basal activity is about 11-fold lower than those of other transglutaminases (e.g., the cytosolic tissue transglutaminase), but reaches levels comparable to those of other transglutaminases on addition of specific surfactant agents. There is no study devoted to understanding the molecular basis of this apparently anomalous activation, which is maximal at ∼1.5 mM sodium dodecyl sulfate. We provide evidence that in the presence of this detergent modifications of the intrinsic fluorescence as well as energy transfer of the protein fluorescence to a micellar probe parallel the activation of the enzyme. As the sodium dodecyl sulfate concentration inducing maximal activation equals the critical micellar concentration, the biological activity of this transglutaminase appears to be modulated by the binding of micellar aggregates. In fact, the enzyme is modified by posttranslational modifications consisting of some lipid tails. At least two of these tails could act as aggregation nuclei of the enzyme with detergents. This behavior is different from that typical of molecular forms purified from other sources.

NoteEffect of a Weak Electrolyte on the Critical Micellar Concentration of Sodium Dodecyl Sulfate☆

AbstractThe critical micellar concentration of sodium dodecyl sulfate is strongly altered bytris(hydroxy-methyl)methylammonium ions. The effect of buffer solutions containing this weak electrolyte as the counterion source has been studied using various concentrations of the acid–base system as well as modifying the pH. Results show that counterion concentrations ranging from 0 to ∼340 × 10−3M induce an appreciable diminution of the critical micellar concentration from ∼8 to ∼0.7 × 10−3M. The analysis of data suggests that the critical micellar concentration of sodium dodecyl sulfate depends on the concentration of weak electrolytes in a way very similar to that of strong electrolytes.

Laparoscopic surgery for gastroesophageal reflux disease during the first year of life☆

AbstractBackground: Very few children need gastroesophageal antireflux surgery during their first year of life; hence, no series has been published so far. The authors report their experience in 3 centers. Methods: From January 1993 to December 1998, 36 infants between 23 days and 13 months of age, suffering from gastroesophageal reflux disease (GERD), underwent surgery by a laparoscopic approach. The patients' weights ranged from 2.4 to 8.5 kg. Preoperative diagnostic studies included esophagograms, manometries, endoscopies, and pH-metries. Fifteen babies (41.6%) had associated anomalies, and 10 (27.7%) were neurologically impaired. Thirty-six laparoscopic fundoplications were performed according to either Toupet's procedure (17 of 36), Rossetti's (10 of 36), Nissen's (8 of 36) or Lortat-Jacob's (1 of 36). Four infants previously had undergone a gastrostomy, whereas 6 needed one during the antireflux procedure. Results: There was no mortality in our series. Three infants (8.3%) had an intraoperative complication: 1 lesion of a diaphragmatic vessel, 1 pneumothorax, and 1 case of severe hiatal hernia requiring conversion to open surgery. During the median follow-up of 22 months, 4 redo procedures were performed (11.1%). Conclusions: This experience shows the feasibility of laparoscopic fundoplication even in children below 1 year of age. An accurate preoperative diagnostic study is mandatory, because 50% of these patients presented associated anomalies. A long and accurate follow-up is necessary to evaluate long-term results and detect possible complications, which can occur as late as 1 year after surgery. In addition, we believe that redo antireflux surgery is possible by the laparoscopic approach without major difficulties, based on our larger experience with older children. J Pediatr Surg 36:715-717. Copyright © 2001 by W.B. Saunders Company.

Original ArticleWork-related upper limb musculoskeletal disorders in paediatric laparoscopic surgery. A multicenter survey

AbstractBackgroundSurgeons are at risk for developing work-related musculoskeletal symptoms (WMS). The present study aims to examine the physical factors and their association with WMS among pediatric laparoscopic surgeons.MethodsA questionnaire consisting of 21 questions was created and mailed to 25 pediatric laparoscopic surgeons (LG). 23/25 surgeons (92%) completed the survey. The questionnaire was analyzed and then split into 2 groups. Group 1 (LG1) included surgeons with greater laparoscopic experience, and group 2 (LG2) included surgeons with less important laparoscopic experience. In addition, we constructed and sent to the same surgeons a similar questionnaire focused on WMS after an open procedure (OG) with the aim to compare results of LG with OG.ResultsThe prevalence rate of WMS with shoulder symptoms was 78.2% in surgeons that performed laparoscopy for more than 10 years, with 60.8% also reporting other pain. In 66.6% this pain is evident only after a long-lasting procedure. Forty-four percent of these surgeons require painkillers at least twice a week. Fifty percent of these surgeons also suffer at home. Fifty-five and one half percent of surgeons indicate that this pain is related to their laparoscopic activity. Forty-three and a half percent think that laparoscopy is beneficial only for the patient but has a bad ergonomic effect for surgeons. Sixty-five and two-tenths percent think that robotic surgery can be helpful to improve ergonomics. Comparing the groups, WMS occur more frequently in LG (78.2%) than in OG (56.5%), but this difference was not statistically significant (χ2 = 0.05). In addition, WMS occur more frequently in LG1 (84.6%) than in LG2 (70%), but this difference was not statistically significant (χ2 = 0.05).ConclusionsThese results confirmed a strong association between WMS and the number of laparoscopic procedures performed. Skilled laparoscopic surgeons have more pain than less skilled laparoscopic surgeons. WMS in the same group of surgeons are more frequent after laparoscopy than after open procedures. The majority of surgeons refer to shoulder symptoms.

Laparoscopic treatment of congenital inguinal hernia in children

AbstractPurpose: The authors report their experience in the laparoscopic treatment of congenital inguinal hernia in children.Methods: Between September 1994 and September 1995, 45 boys between 8 months and 13 years of age (mean, 4 years) were treated laparoscopically for hydroceles, spermatic cord cysts, or hernias. Twenty-six (57.8%) boys showed a right inguinal hernia, 17 (37.8%) a left hernia, and two cases (4.4%) presented the clinical data of a bilateral pathology. The approach used for small hernias was the placement of purse-string suture around the internal orifice of the inguinal canal (28 cases). As to hernias exceeding 4 to 5 mm in diameter, the external hemicircumference of the neck was opened to bring the conjoined tendon closer to the crural arch with a nonresorbable suture (17 cases). There was never need to use a prosthesis.Results: Surgery lasted from 15 to 45 minutes with the duration decreasing with experience. There were no intra- or postsurgical complications. Two patients (4.4%) experienced a recurrent inguinal hernia, which was successfully operated on again with laparoscopy.Conclusion: The early results of these authors suggest that laparoscopic surgery is a feasible and safe technique for the treatment of patent peritoneal vaginal canal (PVC) and inguinal hernia in children.

Pediatric UrologyTHE VALUE OF 2-STEP LAPAROSCOPIC FOWLER-STEPHENS ORCHIOPEXY FOR INTRA-ABDOMINAL TESTES

AbstractPurposeWe report our experience in the treatment of high intra-abdominal testis with a complete laparoscopic 2-stage Fowler-Stephens procedure with associated transperitoneal closure of the internal ring in pediatric patients.Materials and MethodsBetween 1990 and 1997, 100 boys with 105 nonpalpable testes underwent laparoscopy. Laparoscopy showed intra-abdominal testis in 40 cases. In 5 cases when the testis was just proximal to the internal ring, we performed standard orchiopexy. In 35 cases with the testis in the high intra-abdominal position, we performed the Fowler-Stephens procedure with the first stage performed laparoscopically. To date, all 35 testis have undergone the second phase after 6 to 12 months (2 by open technique, 33 by laparoscopy). The last 33 patients underwent the second phase of the 2-stage Fowler-Stephens procedure by laparoscopy with associated video surgical transperitoneal closure of internal ring.ResultsAll testes were successfully placed in the scrotum. At a mean 30 months of followup, with clinical examination, ultrasonography and comparative colorimetric echo Doppler study, all testes were viable in the scrotum, except for 1 that became atrophic 2 months after the second open phase of 2-stage Fowler-Stephens technique.ConclusionsOur early results suggest that the 2-stage Fowler-Stephens procedure, performed completely using laparoscopy, is a feasible technique for treating high intra-abdominal testis.

Original ArticleCyclosporine induces different responses in human epithelial, endothelial and fibroblast cell cultures

Cyclosporine induces different responses in human epithelial, endothelial and fibroblast cell cultures.BackgroundNephrotoxicity, accelerated atherosclerosis, and graft vascular disease are common complications of cyclosporine long-term treatment characterized by a wide disruption of organ architecture with increased interstitial areas and accumulation of extracellular matrix (ECM). How cyclosporine induces these changes is not clear, but it is conceivable that they are the sum of changes induced at the cell level.MethodsWe studied the effects of cyclosporine on human endothelial (HEC), epithelial (HK-2), and fibroblast (MRC5) cells. Cell proliferation was evaluated by cell counting, apoptosis and collagen production by enzyme-linked immunosorbent assay, and nitric oxide by measuring the concentration of nitrite/nitrate in the cell supernatant. (α1)I and (α2)IV collagen, matrix metalloprotease-9 (MMP9), and tissue inhibitors of metalloprotease-1 (TIMP-1) mRNA levels were measured by reverse transcription-polymerase chain reaction. Proteolytic activity was evaluated by zymography.ResultsCyclosporine showed a marked antiproliferative and proapoptotic effect on endothelial and epithelial cells. Fibroblast growth was not affected by cyclosporine. Nitric oxide was up-regulated by cyclosporine in epithelial cells and fibroblasts but not in endothelial cells. (α1)I and (α2)IV collagen synthesis was increased in cyclosporine-treated endothelial and epithelial cells, respectively. Proteolytic activity was increased in endothelial and epithelial cells. TIMP-1 mRNA was up-regulated by cyclosporine in fibroblasts.ConclusionsOur results demonstrate that cyclosporine exhibits an antiproliferative effect on endothelial and epithelial cells. This effect is associated with induction of apoptosis probably via nitric oxide up-regulation in epithelial cell cultures. Cyclosporine treatment induces ECM accumulation by increasing collagen synthesis in endothelial and epithelial cells and reducing its degradation by up-regulating TIMP-1 expression in fibroblasts. We conclude that cyclosporine affects cell types differently and that the disruption of organ architecture is the result of multiple effects at the cell level.

Imaging Teaching CaseRenal Blood Flow Redistribution During Acute Kidney Injury

We describe a case of acute kidney injury with decreased kidney perfusion in which contrast-enhanced computed tomography of the abdomen was performed for a nonrenal indication. This imaging procedure showed intrarenal blood flow redistribution from the cortex to the medulla that reversed after recovery of kidney function. Renal blood flow redistribution was described first almost a century ago in experimental models of renal ischemia, but clinical imaging studies are scarce. We provide a clear example of this phenomenon using contrast-enhanced computed tomography.

Current concepts in the management of inguinal hernia and hydrocele in pediatric patients in laparoscopic era

AbstractThe surgical repair of inguinal hernia and hydrocele is one of the most common operations performed in pediatric surgery practice. This article reviews current concepts in the management of inguinal hernia and hydrocele based on the recent literature and the authors׳ experience. We describe the principles of clinical assessment and anesthetic management of children undergoing repair of inguinal hernia, underlining the differences between an inguinal approach and minimally invasive surgery (MIS). Other points discussed include the current management of particular aspects of these pathologies such as bilateral hernias; contralateral patency of the peritoneal processus vaginalis; hernias in premature infants; direct, femoral, and other rare hernias; and the management of incarcerated or recurrent hernias. In addition, the authors discuss the role of laparoscopy in the surgical treatment of an inguinal hernia and hydrocele, emphasizing that the current use of MIS in pediatric patients has completely changed the management of pediatric inguinal hernias.

Surgical Anatomy of the Mediastinum

After a short description of mediastinal anatomy and embryology, the authors illustrate the division of the mediastinum, based on criteria of either surgical anatomy or radiological anatomy. Lastly, they discuss the topographical distribution of the mediastinal masses in anterior, visceral, and posterior compartments of the mediastinum.

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