Biography:

In the past S Diallo has collaborated on articles with M Adjuik. One of their most recent publications is A capillary gas chromatography/mass spectrometric method for the quantification of hydroxysteroids in human plasma☆. Which was published in journal Analytical Biochemistry.

More information about S Diallo research including statistics on their citations can be found on their Copernicus Academic profile page.

S Diallo's Articles: (2)

A capillary gas chromatography/mass spectrometric method for the quantification of hydroxysteroids in human plasma☆

AbstractA specific and sensitive methodology for the quantitative determination of hydroxysteroids dehydroepiandrosterone and pregnenolone and their main metabolites in human plasma is described. Hydroxysteroids were extracted using methanol and steroids were further separated by reverse-phase high-performance liquid chromatography, allowing for minimization of the possible chromatographic interferences. Eluted fractions were collected, pooled, and analyzed by gas chromatography–mass spectrometry as trimethylsilyl ether derivatives. The quantification was performed with single-ion monitoring of the highly abundant m/z 129 or m/z 358 fragments. The combination of the chromatographic characteristics to the specific fragments ensured the selectivity and specificity of the method. Under these conditions the method was linear (typical R2 is superior to 0.98 for all hydroxysteroids studied) over the concentration range of 2×10−9 to 10−6 M with good precision and accuracy.

Fast track — ArticlesAmodiaquine-artesunate versus amodiaquine for uncomplicated Plasmodium falciparum malaria in African children: a randomised, multicentre trial

SummaryBackgroundIncreasing drug resistance limits the choice of efficacious chemotherapy against Plasmodium falciparum malaria in Africa. Amodiaquine still retains efficacy against P falciparum in many African countries. We assessed the safety, treatment efficacy, and effect on gametocyte carriage of adding artesunate to amodiaquine in three randomised trials in Kenya, Sénégal, and Gabon.MethodsWe enrolled 941 children (400 in Kenya, 321 in Sénégal, and 220 in Gabon) who were 10 years or older and who had uncomplicated P falciparum malaria. Patients were randomly assigned amodiaquine (10 mg/kg per day for 3 days) plus artesunate (4 mg/kg per day for 3 days) or amodiaquine (as above) and placebo (for 3 days). The primary endpoints were parasitological cure rates at days 14 and 28. Analysis was by intention to treat and by an evaluability method.FindingsBoth regimens were well tolerated. Six patients in the amodiaquine-artesunate group and five in the amodiaquine group developed early, drug-induced vomiting, necessitating alternative treatment. By intention-to-treat analysis, the day-14 cure rates for amodiaquine-artesunate versus amodiaquine were: 175/192 (91%) versus 140/188 (74%) in Kenya (Δ=16·7% [95% Cl 9·3–24·1], p<0·0001), 148/160 (93%) versus 147/157 (94%) in Senegal (−1·1% [−6·7 to 4·5], p=0·7), and 92/94 (98%) versus 86/96 (90%) in Gabon (8·3% [1·5–15·1], p=0·02). The corresponding rates for day 28 were: 123/180 (68%) versus 75/183 (41%) in Kenya (27·3% [17·5–37·2], p<0·0001), 130/159 (82%) versus 123/156 (79%) in Sénégal (2·9% [−5·9 to 11·7], p=0·5), and 80/94 (85%) versus 70/98 (71%) in Gabon (13·7% [2·2–25·2], p=0·02). Similar rates were obtained by evaluability analysis.InterpretationThe combination of artesunate and amodiaquine improved treatment efficacy in Gabon and Kenya, and was equivalent in Sénégal. Amodiaquine-artesunate is a potential combination for use in Africa. Further investigations to assess the potential effect on the evolution of drug resistance, disease transmission, and safety of amodiaquine-artesunate are warranted.

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