Biography:

In the past C.P. Price has collaborated on articles with D.D. Woodman and J. Calvin. One of their most recent publications is Brief notesA comparison of two colorimetric procedures for the assay of 5′-nucleotidase. Which was published in journal Clinica Chimica Acta.

More information about C.P. Price research including statistics on their citations can be found on their Copernicus Academic profile page.

C.P. Price's Articles: (7)

Estimation of serum total lipids

AbstractA method is presented which provides a rapid reliable procedure for the estimation of total lipids in serum. Results obtained by this method compare favourably with those obtained by established gravimetric and turbidimetric techniques.

Training and education in clinical biochemistry in the United Kingdom☆

AbstractIn the United Kingdom, clinical biochemistry is practised by medical and non-medical graduates. Their training is postgraduate, led by the profession and has a strong vocational orientation. Although there is considerable overlap between the training of medical and non-medical graduates, each group has a different career structure and different training requirements. The training of non-medical biochemists has recently been restructured. Their new training programme is described in detail; for comparison, the training of medical graduates is outlined.

Novel enzymes as reagents

AbstractEnzymes are now used in a wide range of analytical methods, primarily for the measurement of substrates and as labels in immunoassays. Enzymes from microbial sources are becoming the preferred choice because of ease of extraction, catabolic activities and opportunities for enhancing yields. Protein engineering of bacterial enzymes will also play a role in future enzyme design with respect to improvements in specificity, reaction kinetics and stability.

Evaluation of fluorescence excitation transfer immunoassay for measurement of specific proteins

AbstractFluorescence excitation transfer immunoassay is a suitable technique for the measurement of serum IgG and CRP. The reagents, once reconstituted, are stable for at least 3 months. The method shows no interference due to bilirubin, lipaemia or haemolysis up to high levels. The assay is simple to perform, reliable and offers considerable advantages over manual techniques such as radial immunodiffusion or electroimmunoassay.

Research articleAssessment of the performance of a capture immunoassay for the bone isoform of alkaline phosphatase in serum

AbstractWe report the analytical validation of an immunocapture assay for the bone isoform of alkaline phosphatase in serum. A between batch imprecision of less than 10% was found, being about 8% at the upper limit of the reference range, and with a detection limit of 0.8 IU/1 at 37°C. The crossreactivity of the method with the liver isoform was found to be in the range of 3–13% depending on the method employed. Unexpectedly the correlation of results with a non-immunological method for the quantitation of bone ALP showed significant differences between samples from children and patients with Paget's disease, with an apparent lower level of capture in the case of children. These data suggest that there may be differences in the epitope recognised by the antibody, which may be due to the presence of different forms of bone enzyme in these two populations. The significance of these observations is not clear at this stage.

Plasma cystatin C determinations in a healthy elderly population

AbstractPlasma cystatin C measurement has been previously shown to be a better indicator of changes in glomerular filtration rate (GFR) than plasma creatinine. The available literature on reference intervals for cystatin C concentration encompasses only paediatric and adult populations up to 60 years of age, therefore we set out to determine an elderly reference range. Blood was taken from 401 subjects (65–101 years) and cystatin C and creatinine concentrations measured using commercially available methodologies. The availability of height and weight measurements allowed the additional calculation of predicted creatinine clearances using the Cockcroft and Gault formulae. Whilst no notable gender difference in cystatin C values was observed (female, 1.48 mg/l; male, 1.53 mg/l), concentrations rose with increasing age (60–79 years, 1.39 mg/l; >80 years, 1.70 mg/l). Conversely, there was a significant (P<0.0001) gender difference in creatinine values (female, 99 μmol/l; male, 120 μmol/l) but none between age groups (60–79 years, 105 μmol/l; >80 years, 113 μmol/l). Calculated GFR determinations resulted in a predicted creatinine clearance range of 21–81 ml/min per 1.73 m2 (n=361). There was no significant difference between gender (male, 18–88 ml/min per 1.73 m2; female, 24–69 ml/min per 1.73 m2), but a very significant 20% decrease in predicted GFR per decade. Sex-related reference intervals for creatinine were established (female, 66–149 μmol/l; male, 71–204 μmol/l); whilst age-related reference intervals were established for both cystatin C (60–79 years, 0.93–2.68 mg/l; >80 years, 1.07–3.35 mg/l) and predicted creatinine clearance (60–79 years, 27–89 ml/min per 1.73 m2; >80 years=18–55 ml/min per 1.73 m2). Plasma cystatin C measurement offers a simple, more sensitive screening assay for early changes in GFR and reflects the decreasing GFR that occurs with increasing age.

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