Biography:

In the past Kazuki Ohta has collaborated on articles with Toshiaki Emori and Yukio Hirata. One of their most recent publications is Concomitant secretion of big endothelin and its C-terminal fragment from human and bovine endothelial cells. Which was published in journal Biochemical and Biophysical Research Communications.

More information about Kazuki Ohta research including statistics on their citations can be found on their Copernicus Academic profile page.

Kazuki Ohta's Articles: (4)

Concomitant secretion of big endothelin and its C-terminal fragment from human and bovine endothelial cells

AbstractA specific radioimmunoassay (RIA) for the carboxyl-terminal fragment (CTF) of big porcine endothelin (pET), an intermediate form of pET, was established to characterize big ET-like and its CTF-like immunoreactivity (LI) secreted from cultured bovine and human endothelial cells (EC). The antibody used crossreacted equally with big pET(1–39) and its CTF(22–39), but not with pET(1–21). Serial dilution curves of the culture media from bovine and human EC were parallel to that of standard CTF. Reverse-phase HPLC coupled with RIAs for big ET and ET of the culture media from bovine and human EC revealed essentially the same elution profiles: two major CTF-LI components, one corresponding to big pET(1–39) and the other to its CTF(22–39), in addition to one major ET-LI component corresponding to pET(1–21). The amounts of CTF-LI were almost equal to that of ET-LI on a molar basis. These data suggest that big ET is processed by a putative ET converting enzyme to yield its CTF and the mature ET(1–21) in EC.

Concomitant secretion of big endothelin and its C-terminal fragment from human and bovine endothelial cells

AbstractA specific radioimmunoassay (RIA) for the carboxyl-terminal fragment (CTF) of big porcine endothelin (pET), an intermediate form of pET, was established to characterize big ET-like and its CTF-like immunoreactivity (LI) secreted from cultured bovine and human endothelial cells (EC). The antibody used crossreacted equally with big pET(1–39) and its CTF(22–39), but not with pET(1–21). Serial dilution curves of the culture media from bovine and human EC were parallel to that of standard CTF. Reverse-phase HPLC coupled with RIAs for big ET and ET of the culture media from bovine and human EC revealed essentially the same elution profiles: two major CTF-LI components, one corresponding to big pET(1–39) and the other to its CTF(22–39), in addition to one major ET-LI component corresponding to pET(1–21). The amounts of CTF-LI were almost equal to that of ET-LI on a molar basis. These data suggest that big ET is processed by a putative ET converting enzyme to yield its CTF and the mature ET(1–21) in EC.

Short communicationVasoconstrictor-induced heterologous down-regulation of vascular atrial natriuretic peptide receptor

AbstractLong-term (24 h) pretreatment of cultured rat vascular smooth muscle cells with 100 nM angiotensin and 1 μM vasopressin induced a marked reduction of the maximal binding capacity of atrial natriuretic peptide (ANP) receptors in a fashion similar to that induced by phorbol ester. The down-regulation of the receptors induced by vasoconstrictors and phorbol ester was concomitantly associated with an attenuation of ANP-stimulated cGMP accumulation. These data suggest that vasoconstrictor-induced activation of protein kinase C is involved in the mechanism of heterologous down-regulation of vascular ANP receptors.

Development of Tilted Planetary Drilling System

AbstractThe study deals with development and practical experiment of the tilted planetary drilling system which is suitable for CFRP (Carbon Fiber Reinforced Plastic) drilling. A new drilling technique for CFRP, which called as tilted planetary drilling is invented. In this paper, the principle model of eccentric mechanism of the tilted planetary drilling built. The eccentricity and the tool position are calculated for design of a prototype of the tilted planetary drilling.

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