Highlights•Biosurfactant produced from an adaptive strain C. tropicalis MTCC230 was functionally characterized as surfactin.•It can significantly reduce the surface tension of water from 72 mN/m to 32 mN/m, with the critical micelle concentration (CMC) of 32.5 mg/l.•It was stable over wide range of pH(2–12), temperature (30ºC-90 °C) and salinity(2%–10%).•In sand-pack column experiment ∼39.80% additional oil was recovered (AOR) this shows its direct approach for MEOR in oil reservoirs.