Ontogenetische und funktionelle Entwicklung der Stomata von Hafer (Avena sativa L.)Ontogenetic and Functional Development of the Stomata of Oats (Avena sativa L.)
Review articleOpen access
1971/01/01 Full-length article DOI: 10.1016/S0015-3796(17)31165-4
Journal: Biochemie und Physiologie der Pflanzen
SummaryThe aim of the investigations was to show the ontogenetic development of the stomata of oats and to check, at which stage of development the stomata aquire the ability to open. Thus the young first leaf was exposed to air with decreased CO2-concentration, which has an opening effect on the stomata.Additionally we tried to differentiate active and non-active stomata by application of the vital-dye toluidine blue. Also the first appearance of chlorophyll was stated by fluorescence in ultra-violet light. Only at the apex of the first young leaf we found stomata, which had attained their full length. These stomata showed a turquoise colour after staining with toluidine blue and contained chlorophyll in their guard cells. Only in this stage they were able to open in CO2-diminished air. Stomata, also with full length, but without chlorophyll and with violet colour after staining with toluidine blue did not open. In all ontogenetic developmental stages the guard cells and subsidiary cells were coloured violet like the epidermal cells.
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