A new color reaction for cholesterol assay
Review articleOpen access
Abstract:

AbstractA new iron-cholesterol reaction, based on the Parekh-Jung method of determination of cholesterol, is described. The reagents consist of ferric acetate-uranium acetate and sulfuric acid-ferrous sulfate. Unlike the commonly employed Liebermann-Burchard and ferric chloride-sulfuric acid reactions, the color obtained is stable and is unaffected by instability of reaction temperature or moisture content of the sulfuric acid.The color reaction obeys Beer's law over a wide clinical range of the serum cholesterol. The reaction can be applied to the assay of cholesterol either as part of the direct method of determination or to the cholesterol pre-extracted by employing other techniques. A close correlation with simultaneous measurements by the method of Abell et al. was obtained.A study of the importance of the use of ferrous sulfate in the reagent system is described. The analytical data are discussed with reference to the reported significance of the chromogenicity of the free and ester forms of cholesterol. No effect of the presence of digitonin on the color reaction was noted. The proposed iron-cholesterol reaction is presented as the color reaction of choice for cholesterol assays.

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