Regular ArticleEnzymatic Hydrolysis of Russian-VX by Organophosphorus Hydrolase
Review articleOpen access
1997/12/18 Full-length article DOI: 10.1006/bbrc.1997.7569
Journal: Biochemical and Biophysical Research Communications
AbstractThe Russian-VX (R-VX) is the principle V-type nerve agent in the chemical warfare (CW) arsenal of the Former Soviet Union. We here report the enzymatic hydrolysis of the P-S bond of Russian-VX by organophosphorus hydrolase (OPH) fromPseudomonas diminuta.While the Michaelis constant,Kmfor R-VX (474 μM), was similar to that for VX (434 μM), theVmaxfor R-VX (2.1 μmoles/mg/min) was about four-fold higher compared to that for VX (0.56 μmoles/mg/min). A 50% inhibition in the rate of the enzymatic hydrolysis of R-VX was observed in the presence of 0.5% ethanol, isoamylalcohol, or isopropanol. The presence of acetonitrile, diethylene glycol, or methanol had marginal effects. These results comprise the first demonstration of enzymatic detoxification of R-VX.
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