Regular ArticleCo-dependency of Calcium and Porphyrin for an Integrated Molecular Structure of Peanut Peroxidase: A Circular Dichroism Analysis
Review articleOpen access
1993/07/15 Full-length article DOI: 10.1006/bbrc.1993.1823
Journal: Biochemical and Biophysical Research Communications
AbstractThe contribution of calcium to the structure of cationic peanut peroxidase was examined using ultraviolet/visible and circular dichroism spectroscopies under conditions in which the 2 moles of Ca2+ bound per mole of enzyme were removed. Cadmium and terbium ions were used as substitutes for calcium in the calcium depleted peroxidase and their influence on the protein structure was examined spectroscopically and compared to native and heme depleted enzymes. A role for the calcium ions in maintaining the active conformation of the peroxidase is proposed.
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