Chapter 9 - Isolation of Cellulase Genes From Thermophilies: A Novel Approach Toward New Gene Discovery
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AbstractMicrobial cellulases, a major group of industrial enzymes, have a wide range of applications as far as biotechnological, environmental issues, industrial products, and processes are concerned. Thermophilic bacteria act as a good source for industrial cellulase because of its compatibility during industrial harsh processes, for example, deinking of paper, softening of fabric material, pulp and paper, biostoning and biopolishing of fabric materials, juice, and animal feed, etc. Genome mediated application of thermophilic bacteria in various fields has become a rising fantasy among biotechnologists in the current scenario. The genetic manipulation of thermophilic bacteria may lead to enhanced cellulase production through recombinant technology, an emerging technology towards cellulase gene isolation of thermophilic bacteria and their expression in suitable hosts for enhanced cellulase production. Cellulase gene isolation from various thermophilic bacteria could be done through various approaches, such as a classical approach, whole genome isolation, whole metagenome isolation, and bioinformatics. In this chapter, various methods toward the isolation of cellulase genes from thermophilic bacteria are described which may lead to novel cellulase gene discovery as well as enhanced cellulase enzyme production.

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