B7 - Microimmunofluorescence
Review articleOpen access

Publisher SummaryThis chapter describes the microimmunofluorescence (MIF) procedure for measuring mycoplasma antibody. This application has a number of advantages, especially regarding sensitivity and specificity: test readings are not affected by antibiotics in the serum, the test antigen is standardized, and different antibody classes are measured. The indirect MIF test has several advantageous features: (1) reproducibility can be maintained because it is possible to store a large batch of antigen in aliquots that are frozen so that there should be no variation from one aliquot to another, (2) the test is quick to perform, particularly if slides with antigen spots have been prepared beforehand, and it is relatively easy to read, (3) only small volumes of reagents are required, and (4) estimation of IgG, IgM, or IgA antibodies is accomplished easily by using the appropriate antiglobulins. The indirect MIF test has been used to measure antibodies directed against several mycoplasmas, including Mycoplasmahominis, M. pneumoniae , and M. synoviae of avian origin. It has been particularly useful in seeking serum antibodies to M. genitalium where the sensitivity of the procedure was found to be greater than that of the metabolism-inhibition test.

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