104 - DEXTRAN PRODUCTION BY FREE AND IMMOBILIZED DEXTRANSUCRASE
Review articleOpen access
Abstract:

ABSTRACTDextransucrase is produced by culturing Leuconostoc mesenteroides NRRL-B 512 with continuous sucrose addition. The final enzyme activity of the fermentation supernatant reaches 180 DSU/ml. The enzyme is purified by direct gel permeation chromatography to give a dextransucrase preparation with a 2500 DSU/mg specific activity in 96.4 % yield.Dextransucrase, free and immobilized, is reversibly inhibited by increasing ionic strength.Dextran synthesis is studied, determining the molecular weight distribution at different reaction times. It is found that average molecular weight increases with conversion, the distribution being disperse at the beginning of the reaction and becoming sharper at the end.Dextransucrase has been immobilized by covalent coupling onto amino-Spherosil activated with glutaraldehyde up to 830 DSU/g support. A deviation from the Michaelis behaviour followed by the free enzyme is then observed. This may be attributed to the viscosity gradient resulting from dextran synthesis in the immobilized enzyme microenvironment.

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