Digestion of fibrinogen by trypsin: II. Characterization of the large fragment obtained
Review articleOpen access
AbstractThe large fragment of the fibrinogen molecule produced by short tryptic digestion has been isolated in a homogeneous state. The physico-chemical parameters of the fragment were determined: s = 5.22, D = 5.12 · 10−7, β− = 0.735. From these values and from the results of approach to sedimentation-equilibrium studies an average molecular weight of 95 000 was calculated. The complete amino acid and hexose content of the fragment was also determined. The relationship of the fragments to the parent fibrinogen molecule are discussed.
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