Messenger RNA of mouse immune interferon (MuIFN-γ)
Review articleOpen access
Akira Fuse - No affiliation found
1982/04/29 Full-length article DOI: 10.1016/0006-291X(82)90929-9
Journal: Biochemical and Biophysical Research Communications
AbstractA lysate of Staphylococcus aureus (S. aureus) induced high-titered (103.6 ∼ 104.6 units/ml) mouse immune interferon (MuIFN-γ) in spleen cell cultures. mRNA was extracted from such cells, purified by oligo(dT) cellulose chromatography and sucrose gradient centrifugation, and injected in Xenopus laevis oocytes. Fractions containing RNA of 15 to 16 S gave rise to IFN activity, that was characterized as IFN-γ by virtue of its acid lability, neutralization by antiserum to partially purified MuIFN-γ, and lack of neutralization by antiserum to NDV-induced L-cell IFN.
Request full text