Plantlet formation in callus tissues of Anthurium andraeanum Lind.
Review articleOpen access

AbstractA tissue culture method is described for the vegetative propagation of Anthurium andraeanum Lind. through callus tissue differentiation. The callus tissue was induced from excised embryos and young parts of adult plants, cultivated on a modified Murashige and Skoog's medium supplemented with the cytokinin PBA. Optimum growth of callus tissue was obtained at 25°C in darkness, but the rate of growth was highly variable amongst callus clones obtained from different genotypes. Callus tissue could be subcultured and induced to form adventitious sprouts, especially in light. These sprouts regenerated roots on the basic culture medium. The first Anthurium clones have been transferred to pots and are now growing vigorously in the greenhouse.

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