A simple, large scale method for preparation of plasminogen-free fibrinogen
Review articleOpen access
1972/12/01 Full-length article DOI: 10.1016/0049-3848(72)90040-0
Journal: Thrombosis Research
AbstractA simple method, involving lysine-Sepharose chromatography was developed for large scale preparation of fibrinogen free from detectable plasminogen. After incubation of the preparation with human urokinase under appropriate conditions for over 24 hours at 37°C, no evidence of degradation of the subunits of fibrinogen was detected by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. Moreover, fibrin clots formed by addition of purified bovine thrombin were not lyzed during incubation for more than 72 hours. Large scale preparation of plasminogen-free fibrinogen requires only a smal column and the treatment takes only a short time, so this method seems much better than those used previously, such as Sephadex G-200 gel filtration or precipitation with ethanol-lysine or ethanol-epsilon aminocaproic acid.
Request full text