Glycopeptides isolated from bovine submaxillary mucin☆
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AbstractExtensive pronase digestion of the desialized bovine submaxillary major mucin (BSM) resulted in a mixture of glycopeptides.Separation of the glycopeptides into nine fractions, fractions a to i, was achieved by linear gradient elution at constant pH (2.6) of 0.05–0.2 m pyridine-formate buffer from Dowex 50-X2 (pyridinium form).Glycopeptide b6 was isolated from fraction b by preparative high-voltage paper electrophoresis, and glycopeptides g5and e7 from fractions g and e, respectively, by preparative high-voltage paper electrophoresis and preparative paper chromatography, in succession.Homogeneity of these glycopeptides was shown by high-voltage paper electrophoresis at pH 1.9, 3.7, and 6.5 and by paper chromatography.The results of the N-terminal analysis by dansylation method with or without sequential Edman degradation and the analytical data before and after a β-elimination reaction of glycopeptides b6, g5, and e7 indicated the structures of these glycopeptides as follows: Glycopeptide b6, seryl-(O-α-N-acetylgalactosaminyl-)-serine; glycopeptide g5, glycyl-(O-α-N-acetylgalactosaminyl-)-serine; glycopeptide e7, seryl-(O-α-N-acetylgalactosaminyl-)-threonyl-glycyl-(O-α-N-acetylgalactosaminyl-)-serine.

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