Automated, isocratic separation of phenylthiohydantoin-amino acids by tandem reverse phase high-pressure liquid chromatography columns☆
Review articleOpen access
Abstract:

AbstractQuantitative separation of the phenylthiohydantoin derivatives of all 20 naturally occurring amino acids is described utilizing two tandem C18 columns and an isocratic elution buffer system. The separation was performed in 35 min. In conjunction with a Spectra-Physics SP8000 liquid chromatograph programmed for “peak-picking” sample collection and autoinjection, unattended, overnight analysis of multiple sequencer steps can be performed.

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