A simple regeneration protocol from stem explants of Jatropha curcas—A biodiesel plant
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AbstractA simple, rapid and cost effective protocol has been developed for high frequency regeneration using stem segments of elite genotypes (CSMCRI-I, CSMCRI-II and CSMCRI-III) of Jatropha curcas. Shoot bud induction (10–15 buds per explant) was achieved on Murashige and Skoog's (MS) medium supplemented with 1.0 ml−1 benzylaminopurine (BAP) in combination with 1.0 mg l−1 6-furfurylamino purine (KN). Stem explant of CSMCRI-II showed highest response (65.3%) followed by CSMCRI-I and CSMCRI-III. These shoot buds developed into shoots when subcultured on MS medium supplemented with 0.5 mg l−1 BAP and 1.0 mg l−1 IAA (indole-3-acetic acid). Shoots of 4.0–5.0 cm length were harvested and cultured on MS medium containing different concentrations of indole-3-butyric acid (IBA) and 40% rooting was achieved in 0.1 mg l−1 IBA after 5 weeks in all the genotypes used. For direct rooting, shoots of 4.0–5.0 cm length were used and rooting was achieved by dipping the base of shoots in MS medium supplemented with 0.1 mg l−1 IBA and 3.5% sodium alginate matrix and subsequently dropping in polymerization medium containing 2.0% calcium chloride. Encapsulated shoots were transferred in polybags filled with sterile soil wetted with sterile distilled water containing 0.5% broad-spectrum fungicide (Bavistine). Rooting could be achieved in 62% of shoots within 3 weeks. Rooted plantlets were successfully hardened and transferred to green house with 92% establishment.

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