16 “Smart” Genetic Screens
Review articleOpen access
1998/01/01 Full-length article DOI: 10.1016/S0580-9517(08)70337-4
Publisher SummaryThis chapter provides an overview of smart genetic screens. The application of screens allows, in general, the assignment of genetic interactions among two or more gene products. This may reflect a direct physical interaction of the two gene products or more often a functional interaction without actual physical contact. The ease of manipulation and the combination of genetic, molecular, and biochemical methods have given yeast several advantages in its use as a valuable model organism for studying fundamental eukaryotic processes. The powerful genetic approaches that can be carried out in yeast have proven useful for unraveling complex cellular aspects that are inherently more difficult to address by strictly biochemical approaches. The chapter describes the most common examples of classical and modern genetic screens and selections to study the function of a given gene, or to identify its interacting partners. It describes genetic screens most commonly used for S. cerevisiae and genetic screens used to identify new genes and their functions. .
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