The binding of 5 s ribosomal ribonucleic acid to ribosomal subunits☆
Review articleOpen access

AbstractThe 75 s ribosomes of the aquatic fungus, Blastocladiella emersonii dissociate at 10−4 m-Mg2+ into 63 s and 45 s subunits. Only the 63 s subunit contains 5 s rRNA, about one molecule per subunit being present. When these subunits are treated with EDTA, the 63 s particle is unfolded to a 47 s particle without extensive release of protein but with the release of 5 s rRNA. Using 32P-labeled 5 s rRNA, we have demonstrated that 5 s rRNA will exchange with the 5 s rRNA on the 63 s subunit. Binding studies demonstrate that 2 molecules of 5 s rRNA will bind to ribosomal subunits or to the isolated 47 s large subunit. Binding to one of the two sites is displaced by transfer RNA.

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