An ultrastructural analysis of microsomal preparations from Musca domestica and Prodenia eridania☆
Review articleOpen access
Abstract:

AbstractThe ultrastructure of abdomen, gut, and fat body microsomal preparations from adult Musca domestica and larval Prodenia eridania was examined by electron microscopy. The components of these enzyme preparations are similar to preparations from mammalian liver and contain membranous vesicles, broken membranes, and RNP particles. Clusters of glycogen granules are a prominent feature of the Prodenia gut preparations. Microbial contaminants are retained in pellets from gut and fat body homogenates. A preliminary centrifugation at 10,000 g for 15 min which removed all mitochondria from M. domestica preparations was inadequate for this purpose when Prodenia gut was being used. The fine structure was intact in Musca and Prodenia gut microsomal pellets. The structural integrity of fat body microsomes was impaired compared to those from the gut when both preparations were made in Millonig's sodium phosphate-sucrose buffer. This difference between Prodenia gut and fat body tissues was increased further when potassium phosphate buffer was the homogenizing medium. It is apparent that buffering systems and centrifugation speeds must be varied from one insect to another and from one tissue to another of the same insect.

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