Selection of reliable reference genes for gene expression studies using quantitative real-time PCR in navel orange fruit development and pummelo floral organs
Review articleOpen access
Abstract:

Highlights•We firstly validated appropriate reference genes during fruit ripening stages of Citrus by qRT-PCR.•CsGAPDH and CsUBL5 were the best combination for all the three experimental sets.•The selection of appropriate reference genes is crucial for accurate quantification of target genes and the best suitable reference genes differed depending on the tissues or experimental conditions.

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