Research reportDifferential immunofluorescent staining of polymorphonuclear leucocytes for discriminating between surface bound and internalised immunoglobulin
Review articleOpen access

AbstractDiscrimination between immunoglobulin on the surface of polymorphonuclear leucocytes and immunoglobulin internalised as a result of phagocytosis has been achieved by direct immunofluorescent staining with fluorescein and rhodamine antiglobulin conjugates of identical specificity applied sequentially to live cells in suspension and after fixation on microscope slides. Surface bound antigen is recognised by double staining whereas internalised material is stained only by the conjugate applied to the fixed preparation. This differential staining procedure was evaluated in a model system in which normal PMNs were exposed to small (< 20 s) and large (> 90 s) aggregates of human IgG.

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