Whole genome amplification: abundant supplies of DNA from precious samples or clinical specimens
Review articleOpen access
Abstract:

AbstractWhole genome amplification methods are used to generate the large amounts of DNA that are required for genetic testing. Preparation of genomic DNA from clinical samples is a bottleneck in high-throughput genotyping and is frequently limited by the amount of specimen available. Precious DNA collections used for association and linkage analysis can be a nonrenewable resource available to only a limited number of laboratories. To address these needs, amplified DNA is now being used in a growing number of research and diagnostic applications. A new method, called multiple displacement amplification, dramatically improves the high-fidelity reproduction of genomic DNA, with 10–100 kb amplified DNA products providing uniform coverage of genes.

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