The importance of set up time and temperature in real—time PCR; an essential reminder
Review articleOpen access
Abstract:

Highlights•PCR can produce non-specific traces if left at room temperature for over 30 min.•This can lead to negative clinical samples being reported as weak false positives.•No effect on control Ct value however significant reduction of control fluorescence.•We suggest monitoring control fluorescence as well as Ct to detect any issues.

Request full text

References (0)

Cited By (0)

No reference data.
No citation data.
Advertisement
Join Copernicus Academic and get access to over 12 million papers authored by 7+ million academics.
Join for free!