Comparison of three containers used for the transport of cooled stallion semen
Review articleOpen access

AbstractThree containers commonly used to transport cooled equine semen (Equitainer,™ ExpectaFoal™ and a Swedish-designed semen-transport container, previously called the Salsbro Box and now called Equine Express™) were compared, using four ejaculates from each of three stallions. Each ejaculate was diluted to a spermatozoal concentration of 25 × 106/ml with a nonfat dry milk-glucose extender containing amikacin sulfate (1 mg/ml) and potassium penicillin G (1000 units/ml). Extended semen was divided into three 40-ml aliquots for placement in each of the three semen-transport containers. The extended semen was stored in the containers for 24 h prior to analysis. Stored semen was warmed for 15 min at 37 °C, then video records of sperm motility were obtained for evaluation using a Hamilton-Thorne motility analyzer equipped with a stage warmer set at 37 °C. The temperature of 40-ml aliquots of semen extender stored in each container was also measured for 60 h using a copper-constantan thermocouple placed in the center of the stored samples.Intervals from onset of storage until sample temperature exceeded 10 °C during the warming phase were 27.5, 33.5 and 53 h, for the Expecta-Foal,™ Equine Express™ and Equitainer,™ respectively. Semen extender stored in the Equitainer™ compared most favorably to ideal cooling rates and storage temperatures published previously. Following a 24-h storage period, the mean percentages of motile, progressively motile, and rapidly motile spermatozoa, as well as the mean spermatozoal curvilinear velocity were similar (P > 0.05) among the three containers.

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