Chapter 12 Induction of Ciliogenesis in Oviduct Epithelium
Review articleOpen access
1995/01/01 Full-length article DOI: 10.1016/S0091-679X(08)60793-1
Publisher SummaryThe purpose of this chapter is to describe a method for inducing ciliogenesis in a model of arrested avian oviduct development. In the developing oviduct, estrogens influence both epithelial proliferation and differentiation. During the differentiation phase, the cellular composition of the luminal epithelium is modified in response to hormonal influence. Initially, the epithelium is characterized by a predominance of ciliated cells that are found in the female genital tract of all vertebrates. In a second step, the characteristic oviduct epithelium consists of ciliated and secretory cells, forming, in most cases, a mucociliary epithelium as in airways of the respiratory tract. In birds, only the left ovary and oviduct develop. The luminal epithelium is composed of a single layer of cuboidal cells that are undifferentiated with a primary cilium. Ciliary beat is oriented from the infundibulum to the uterus, and the determination of ciliary polarity is established before differentiation. Ciliogenesis occurs in the same basic pattern in oviduct cells of mammals and birds. Centrioles move under the cellular surface and attach to the apical plasma membrane that has previously developed microvilli. Centrioles are then called basal bodies, from which the cilia grow at the cell surface by lengthening of the axonemal microtubules. According to the nature and dose of hormones and the duration of treatment, development is oriented toward either the ciliogenic or the secretory path of epithelial differentiation. Estrogen treatment induces ciliogenesis; undifferentiated epithelium turns into both undifferentiated cells and cells undergoing ciliogenesis. Progesterone hyperstimulation in association with estrogen treatment strongly induces the secretory process and inhibits the differentiation of ciliated cells. The balance between estrogen and progesterone is critical for harmonious development of the oviduct. The chapter details the methods, ovariectomy, and hormonal treatment.
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