[32] Use of antisense oligonucleotides to study Rab function in Vivo
Review articleOpen access

Publisher SummaryThe use of antisense oligonucleotides provides a useful tool for studying expression of small GTP-binding proteins in tissue culture. It is still extremely difficult to knock out a gene in vitro. Thus, transient inhibition of protein expression by antisense oligonucleotides might be very useful for some biological questions. The possibility to create null phenotypes in cells that are exposed to antisense oligonucleotides immediately after plating provides a very promising approach. Several different approaches have been used in the chapter to modify normal oligomers: the modification of the phosphodiester linkage, attachment of additional groups, and attachment of chemically active groups. Phosphorothioate oligonucleotides have been shown to be effective in selectively inhibiting expression of a variety of genes, including Ras p2114, and have been considered best for depleting neurons of Rab8p.

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