Designing a nine cysteine-less DNA packaging motor from bacteriophage T4 reveals new insights into ATPase structure and function
Review articleOpen access

Highlights•The packaging motor of bacteriophage T4 translocates DNA up to 2000 bp/s.•Functional packaging motors lacking all nine cysteines were designed.•Single mutant motors translocating fluorescent DNA are imaged in real time.•A hydrophobic residue after catalytic glutamate of Walker B motif is essential.•The Walker B motif may be redefined as β-strand-DE-Z (hydrophobic amino acid).

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