Join Copernicus Academic and get access to over 12 million papers authored by 7+ million academics.

Related Articles:

• Thionoesters: A Native Chemical Ligation-Inspired Approach to Cysteine-Triggered H2S Donors.

• Side-chain cysteine-functionalized poly(2-oxazoline)s for multiple peptide conjugation by native che Read more...

• Combination of Thiol-Additive-Free Native Chemical Ligation/Desulfurization and Intentional Replacem Read more...

• Native Chemical Ligation Strategy to Overcome Side Reactions during Fmoc-Based Synthesis of C-Termin Read more...

• Revisiting ligation at selenomethionine: Insights into native chemical ligation at selenocysteine an Read more...

A cysteine-appended deoxyuridine for the postsynthetic DNA modification using native chemical ligation

Shuji Takeda - No affiliation found

2005/03/28 Short communication DOI: 10.1016/j.tetlet.2005.02.018 Journal: Tetrahedron Letters

Abstract:

AbstractA new deoxyuridine derivative 6 bearing a cysteine group at the C5 position was synthesized and incorporated into oligodeoxynucleotide (ODN) by phosphoramidite chemistry. The postsynthetic DNA modification of the cysteine-containing ODN using native chemical ligation with thioesters of biotin and green fluorescent protein variant was successfully demonstrated.