Research lettersReoxidation and renaturation studies on the main toxin of Naja naja samarensis
Review articleOpen access
J.J. Gacond - No affiliation found
1984/12/03 Short communication DOI: 10.1016/0014-5793(84)81252-1
Journal: FEBS Letters
AbstractThe main toxin of Naja naja samarensis is a very small and rigid protein (M, 6850, 8 cysteines). When fully reduced, it regains its native conformation by a mechanism involving a rapid cysteine oxidation and a slower, less temperature-dependent disulfide exchange. In a native-like form of the protein we observed a population whose cysteines were incompletely reoxidized. When intermediates with blocked cysteines were incubated with oxidized and reduced glutathione, the percentage of native-like molecules increased. These findings suggest a multiple folding pathway.
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