Effects of cyclic nucleotides on the shedding of tumor cell surface membranes☆
Review articleOpen access

AbstractMastocytoma P815 cells can be induced to shed membrane vesicles (MV) from their surfaces by low temperature [24], a condition known to disrupt microtubules. In the present study we have investigated the effects of various nucleotides on this tumor cell surface behaviour. Our data show that the cyclic nucleotides cAMP and cGMP significantly inhibit the low temperature-induced shedding of MV from the tumor cell surfaces. GTP and the phosphodiesterase inhibitor theophylline exhibited similar inhibitory effects. These data are interpreted in terms of nucleotide-induced stabilization of cytoskeletal components. Transmission (TEM) and scanning electron microscopy (SEM) of cells shedding MV revealed that these structures originated from blebs on the cell surface.

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