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Cross-reacting surface antigens on Leishmania promastigotes

R. Ramasamy - No affiliation found

1983/08/01 Full-length article DOI: 10.1016/S0020-7519(83)80037-X Journal: International Journal for Parasitology

Abstract:

AbstractThree strains of Leishmania donovani from America, India and Kenya and a strain of Leishmania mexicana were grown in culture as promastigotes. The parasites werw surface labelled by lactoperoxide-catalysed iodination and the surface proteins analysed by polyacrylamide gel electrophoresis and autoradiography. The four strains possessed surface proteins of similar mol.wts. including a 59,000 mol.wt. glycoprotein. Immune-precipitation using rabbit anti-Leishmania sera revealed that the 59,000 mol.wt. protein and other proteins on the surfaces of the promastigotes cross-reacted antigenically. Common surface antigens on the four strains was also shown by immunofluorescence studies using the rabbit anti-Leishmania sera and monoclonal antibodies raised against one of the strains.